Assessment of the informativeness of iPBS markers for identifying and differentiating Ukrainian hazelnut varieties

Mishchenko, A. M.; Andreev, I. O.; Kunakh, V. A.

doi:10.7124/bc.000B2A

Biopolym. Cell. 2025; 41(4):266.

http://dx.doi.org/10.7124/bc.000B2A

Molecular and Cell Biotechnologies

Assessment of the informativeness of iPBS markers for identifying and differentiating Ukrainian hazelnut varieties

1Mishchenko A. M., 1Andreev I. O., 1Kunakh V. A.

Institute of Molecular Biology and Genetics, NAS of Ukraine
150, Akademika Zabolotnoho Str., Kyiv, Ukraine, 03143

Abstract

Aim. To assess the informativeness of iPBS markers for the identification and differentiation of Ukrainian hazelnut varieties. Methods. Thirty Corylus spp. specimens (25 varieties and 5 wild plants) were analyzed using PCR amplification with 24 iPBS primers. Informativeness indices were calculated based on PCR banding profiles. Results. Six primers with the highest polymorphism were selected. They produced 95 bands (15.8 per primer on average), only one of which was monomorphic. Mean resolving power (Rp) of these primers was 6.760 and discriminating power (D_L) 0.948, while the number of non-differentiated pairs (ND) averaged 3.2 per primer. Primer 2402 distinguished all varieties, and two other primers were unable to differentiate between only one pair. In comparison with SSR markers, iPBS demonstrated higher Rp and D_L and significantly lower ND values. Conclusions. iPBS markers are capable of providing an efficient and reliable tool for the differentiation of varieties and can be successfully applied in hazelnut breeding programs.

Keywords: Corylus spp., hazelnut, variety, breeding, iPBS markers

Full text: (PDF, in English)

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